The Enzo Ferrari is a 12 cylinder mid-engine berlinetta named after the company's founder, Enzo Ferrari. It was built in 2002 using Formula One technology, such as a carbon-fibre body, F1-style electrohydraulic shift transmission, and Carbon fibre-reinforced Silicon Carbide (C/SiC) ceramic composite disc brakes. Also used are technologies not allowed in F1 such as active aerodynamics and traction control. After a downforce of 775 kg (1,709 lb) is reached at 355.6 km/h (221 mph) the rear wing is actuated by computer to maintain that downforce.

The Enzo's V12 engine is the first of a new generation for Ferrari. It is based on the architecture of the V8 found in sister-company Maserati's Quattroporte, using the same basic architecture and 104 mm (4.1 in) bore spacing. This design will replace the former architectures seen in V12 and V8 engines used in most other contemporary Ferraris. The 2005 F430 is the second Ferrari to get a version of this new powerplant.

The Enzo was initially announced at the 2002 Paris Motor Show with a limited production run of 349 and at US $659,330. The company sent invitations to existing customers, specifically, those who had previously bought the F40 and F50. All 349 cars were sold in this way before production began. Later, after numerous requests, Ferrari decided to build 50 more Enzos, bringing the total to 399. Before being unveiled at the Paris Motor show, the Enzo (that was used in the show) was flown from Italy to California to be filmed in Charlie's Angels: Full Throttle. It was driven on a beach by actress Demi Moore. After filming was complete, the Enzo was flown to France to be in the Motor Show. Enzos are listed as being built in 2003.

Ferrari built one more Enzo — the 400th car — and it was auctioned by Sotheby's Maranello Auction on June 28, 2005, to benefit survivors of the 2004 Tsunami for €950,000 (US$1,274,229), almost twice its list price. This sum was presented to Pope Benedict XVI, while former Ferrari Formula One driver Michael Schumacher gave the pope a steering wheel to commemorate the donation. This wheel included a plaque which read, "The Formula 1 World Champion's steering wheel to His Holiness Benedict XVI, Catholicism's driver."

The Enzo Ferrari typically trades above $1,000,000 (£500,000) at auction.

Three prototype "mules" were built, M1, M2, and M3. Each was bodied to look like a 348, even though the mules were built in 2000. The third mule was offered for auction alongside the 400th Enzo in June, 2005, bringing €195,500 (US$236,300).

As the result of the Enzo, Ferrari has decided to use some of the technology developed for it in a small-scale program to get more feedback from certain customers for use in future car design as well as their racing program. The core of this program is the Ferrari FXX. It is loosely based on the Enzo's design with a highly-tuned 6.3 litre version of the Enzo's engine putting out roughly 800 PS (590 kW; 790 hp). The gearbox is new as well as the tires (custom-designed for this car by Bridgestone) and the brakes (developed by Brembo). In addition, the car is fitted with extensive data-recording and telemetry systems to allow Ferrari to record the car's behavior. This information will be used by Ferrari to develop their next sports car.

Like the Enzo, the car was sold to specially selected existing clients of Ferrari only; the initial price was €1.3 million. Unlike the Enzo, the clients did not take delivery of the car themselves. Rather, it is maintained by Ferrari and available for the client's use on various circuits as arranged by Ferrari and also during private track sessions. The car is not expected to be street-legal or suitable for road use.

The Ferrari FXX program will continue until 2008/2009 with the Ferrari FXX Evoluźione. The car will continue to be improved under the Evolution kit, which will continually adjust specifics to create more power, change gearing, and remove drag. The 6262 cc V12 engine will be pushing out 860 PS (630 kW; 850 hp) at 9500 rpm. There will be gearbox changes so that shift time will be reduced to 60 milliseconds per shift, a reduction of 20 milliseconds. The car will also undergo aerodynamic changes and improvements to the traction control system. Its top speed is 227 miles per hour (365 km/h).